Lab of Cancer Angiogenesis and Nanomedicine
Setting up murine models of breast, ovarian and prostate cancer, osteosarcoma, brain tumors and melanoma for determination of tumor growth and metastasis: Tumor cell inoculation (subcutaneous, dermal, intracranial, intracardiac, intra-tibia, intra-mammary, pancreatic, adrenal gland, prostate), preparation of fluorescently-labeled and luciferase-expressing tumor cells.
Imaging: Whole animal imaging (Maestro, IVIS, Biospace, US and CellVizio) and molecular imaging (Confocal).
Motility, invasion, migration, adhesion and trans-endothelial migration assays, for determination of cell chemotaxis: Transwells and scratch assays.
Transfection (siRNA and DNA) and infection of vectors to established cell lines.
ELISA, quantitative real-time PCR, Western blotting, Confocal microscopy, e.g. for determination of intracellular and extracellular factors.
Flow cytometry and cell sorting, for determination of cell surface and intracellular molecules in cells.
MTT proliferation and cell viability (coulter counter) assays.
Immunochemistry staining of tumor biopsy sections of patients (H&E, CD31, apoptosis, proliferation, etc.).
Prof. Ronit Satchi-Fainaro, Ph.D.
Head, Cancer Angiogenesis and Nanomedicine Laboratory,
Sackler Faculty of Medicine, , Tel Aviv University, Tel-Aviv 69978, Israel
P. +972.3.6407427 (Office); +972.3.6408733 (Lab) | F. +972.3.6409113 | E. email@example.com