We used plasmids in any biotechnological objective (both in the academia and in the industry) and in all model organisms. For example, plasmids are used in fields such as FoodTech, pharma, agriculture, etc. Currently, researchers chose between two possibilities: high copy plasmid (e.g. 500 copies) and low copy plasmid (e.g. 10 copies). However, the ability to generate a plasmid with the exact required copy number (e.g. any value between 1-600) could significantly improve most of the biotechnological solutions. For example, if the objective is maximizing the protein production of a heterologous protein a too low copy number will of course result in very low protein levels; however, a too high copy number will introduce a burden to the cell and thus will also decrease the protein levels. Thus, there is a copy number which is optimal for this objective. We suggest a novel tool for designing plasmid copy numbers in various organisms based on a unique set of algorithms. This tool should be relevant to any researcher or company which is working with plasmids.

UNMET NEED
Choosing the copy number of your plasmid that will optimize your needs.

OUR SOLUTION
Models and algorithms for designing plasmid copy numbers.

APPLICATIONS
Anyone who works with bacterial plasmids: FoodTech, pharma, vaccine design, agriculture, research, and more.

STATUS
Ready to use. The models were validated.

INTELLECTUAL PROPERTY
A patent and know-how.

REFERENCES
1. Rinat Saban, Ofri Tfilin, David Haggiag, Matan Arbel, Tamir Tuller. Publications Deciphering Plasmid Replication Dynamics: A Computational Approach to Predict ColE1-like Plasmids Copy Number. Under review